Question:
I think this means that pycnogenol(Pine Bark extract) fights Interferon Gamma induced inflammation . Which sound good to me. See you all in the forest. Jack P.S. Hint — They are the trees with the needles… 1: Free Radic Biol Med 2000 Jan 15;28(2):219-27Related Articles, Books Pine bark extract pycnogenol downregulates IFN-gamma-induced adhesion of T cells to human keratinocytes by inhibiting inducible ICAM-1 expression. Bito T, Roy S, Sen CK, Packer L. Department of Molecular and Cell Biology, University of California, Berkeley 94720-3200, USA. Expression of intercellular adhesion molecule-1 (ICAM-1) is necessary for leukocyte/keratinocyte interactions. Upregulation of ICAM-1 expression in keratinocytes has been observed in several inflammatory dermatoses, such as psoriasis, atopic dermatitis, and lupus erythematosus. Inflammatory cytokines, such as interferon-gamma (IFN-gamma), upregulate ICAM-1 expression in keratinocytes. Because of potent antioxidant and anti-inflammatory properties of the French maritime pine bark extract, Pycnogenol (Horphag Research, Geneva, Switzerland), its effects were investigated on the interaction of T cells with keratinocytes after activation with IFN-gamma and the molecular mechanisms involved in such interactions. Studies were performed using a human keratinocyte cell line, HaCaT. Cell adhesion in the presence of IFN-gamma was studied using a coculture assay. Treatment of HaCaT cells with 20 U/ml IFN-gamma for 24 h markedly induced adherence of Jurkat T cells to HaCaT cells. PYC pretreatment (50 microg/ml, 12 h) significantly inhibited IFN-gamma induced adherence of T cells to HaCaT cells (p < .01). ICAM-1 plays a major role in the IFN-gamma-induced adherence of T cells to keratinocytes. Thus, the effect of PYC on IFN-gamma-induced ICAM-1 expression was investigated as well. Pretreatment of HaCaT cells with PYC significantly inhibited IFN-gamma-induced expression of ICAM-1 expression in HaCaT cells. The downregulation of inducible ICAM-1 expression by PYC was both dose and time dependent. A 50 microg/ml dose of PYC and a 12 h pretreatment time (i.e., before activation with IFN-gamma) provided maximal (approximately 70%) inhibition of inducible ICAM-1 expression in HaCaT cells. Gamma-activated sequence present on the ICAM-1 gene confers IFN-gamma responsiveness in selected cells of epithelial origin (e.g., keratinocytes) that are known to express ICAM-1 on activation with IFN-gamma. Gel-shift assays revealed that PYC inhibits IFN-gamma-mediated activation of Stat1, thus suggesting a transcriptional regulation of inducible ICAM-1 expression by PYC. These results indicate the therapeutic potential of PYC in patients with inflammatory skin disorders.
Response:
Idaknow, some guy in a health food store tried to sell me the stuff, gave a free book too…….I gave him the excuse that I couldn’t take anything without my doc’s permission…….I was in there buying boxes and boxes of herbal tea…….that’s as about "herbal" as I’m able to get. All I know is that when I used to get a real Christmas tree, most of them affected my breathing, they irritate my throat when in my home, so the thought of INGESTING a pine-needle based "alternative med/herb" convinced me that I would certainly croak! "carmel" <car…@cyberwizards.com.au> wrote in message
news:3B636C5C.847C8D2C@cyberwizards.com.au… – Hide quoted text — Show quoted text -> What if you’re scared of needles? > Carmel > jack n dalton wrote: > > I think this means that pycnogenol(Pine Bark extract) fights Interferon > > Gamma induced inflammation . Which sound good to me. See you all in the > > forest. > > Jack > > P.S. Hint — They are the trees with the needles… > — > "Don’t wait for a light to appear at the end of the tunnel. > Stride down there and light the bloody thing yourself!" > Web sites at http://www.jaragun.com/ > http://www.geocities.com/peripata/
Response:
"jack n dalton" <jdal…@ix.netcom.com> wrote in message news:9jvi33$6h7$1@slb7.atl.mindspring.net… > I think this means that pycnogenol(Pine Bark extract) fights Interferon > Gamma induced inflammation . Which sound good to me. See you all in the > forest. > Jack > P.S. Hint — They are the trees with the needles…
Don’t forget to take your burner, solvents and separator with you, Jack. Gotta be careful you don’t end up just sucking turpentine outta that bark. Ask any old studio painter in oils what that stuff does to your liver and your thinking. <G> — ((((((((((U)))))))))) Michael <muirh…@island.net> -=| Livin’ on Island Time |=- – Hide quoted text — Show quoted text -> 1: Free Radic Biol Med 2000 Jan 15;28(2):219-27Related Articles, > Books > Pine bark extract pycnogenol downregulates IFN-gamma-induced adhesion of > T cells to human keratinocytes by inhibiting inducible ICAM-1 > expression. > Bito T, Roy S, Sen CK, Packer L. > Department of Molecular and Cell Biology, University of California, > Berkeley 94720-3200, USA. > Expression of intercellular adhesion molecule-1 (ICAM-1) is necessary > for leukocyte/keratinocyte interactions. Upregulation of ICAM-1 > expression in keratinocytes has been observed in several inflammatory > dermatoses, such as psoriasis, atopic dermatitis, and lupus > erythematosus. Inflammatory cytokines, such as interferon-gamma > (IFN-gamma), upregulate ICAM-1 expression in keratinocytes. Because of > potent antioxidant and anti-inflammatory properties of the French > maritime pine bark extract, Pycnogenol (Horphag Research, Geneva, > Switzerland), its effects were investigated on the interaction of T > cells with keratinocytes after activation with IFN-gamma and the > molecular mechanisms involved in such interactions. Studies were > performed using a human keratinocyte cell line, HaCaT. Cell adhesion in > the presence of IFN-gamma was studied using a coculture assay. Treatment > of HaCaT cells with 20 U/ml IFN-gamma for 24 h markedly induced > adherence of Jurkat T cells to HaCaT cells. PYC pretreatment (50 > microg/ml, 12 h) significantly inhibited IFN-gamma induced adherence of > T cells to HaCaT cells (p < .01). ICAM-1 plays a major role in the > IFN-gamma-induced adherence of T cells to keratinocytes. Thus, the > effect of PYC on IFN-gamma-induced ICAM-1 expression was investigated as > well. Pretreatment of HaCaT cells with PYC significantly inhibited > IFN-gamma-induced expression of ICAM-1 expression in HaCaT cells. The > downregulation of inducible ICAM-1 expression by PYC was both dose and > time dependent. A 50 microg/ml dose of PYC and a 12 h pretreatment time > (i.e., before activation with IFN-gamma) provided maximal (approximately > 70%) inhibition of inducible ICAM-1 expression in HaCaT cells. > Gamma-activated sequence present on the ICAM-1 gene confers IFN-gamma > responsiveness in selected cells of epithelial origin (e.g., > keratinocytes) that are known to express ICAM-1 on activation with > IFN-gamma. Gel-shift assays revealed that PYC inhibits > IFN-gamma-mediated activation of Stat1, thus suggesting a > transcriptional regulation of inducible ICAM-1 expression by PYC. These > results indicate the therapeutic potential of PYC in patients with > inflammatory skin disorders.
Response:
But it SMELLS so good!!!! 
Ahhh, me old turps-sniffing days…….. Carmel – Hide quoted text — Show quoted text -Michael wrote: > "jack n dalton" <jdal…@ix.netcom.com> wrote in message > news:9jvi33$6h7$1@slb7.atl.mindspring.net… > > I think this means that pycnogenol(Pine Bark extract) fights Interferon > > Gamma induced inflammation . Which sound good to me. See you all in the > > forest. > > Jack > > P.S. Hint — They are the trees with the needles… > Don’t forget to take your burner, solvents and separator with you, Jack. Gotta > be careful you don’t end up just sucking turpentine outta that bark. Ask any > old studio painter in oils what that stuff does to your liver and your thinking. > <G> > — > ((((((((((U)))))))))) > Michael <muirh…@island.net> > -=| Livin’ on Island Time |=- > > 1: Free Radic Biol Med 2000 Jan 15;28(2):219-27Related Articles, > > Books > > Pine bark extract pycnogenol downregulates IFN-gamma-induced adhesion of > > T cells to human keratinocytes by inhibiting inducible ICAM-1 > > expression. > > Bito T, Roy S, Sen CK, Packer L. > > Department of Molecular and Cell Biology, University of California, > > Berkeley 94720-3200, USA. > > Expression of intercellular adhesion molecule-1 (ICAM-1) is necessary > > for leukocyte/keratinocyte interactions. Upregulation of ICAM-1 > > expression in keratinocytes has been observed in several inflammatory > > dermatoses, such as psoriasis, atopic dermatitis, and lupus > > erythematosus. Inflammatory cytokines, such as interferon-gamma > > (IFN-gamma), upregulate ICAM-1 expression in keratinocytes. Because of > > potent antioxidant and anti-inflammatory properties of the French > > maritime pine bark extract, Pycnogenol (Horphag Research, Geneva, > > Switzerland), its effects were investigated on the interaction of T > > cells with keratinocytes after activation with IFN-gamma and the > > molecular mechanisms involved in such interactions. Studies were > > performed using a human keratinocyte cell line, HaCaT. Cell adhesion in > > the presence of IFN-gamma was studied using a coculture assay. Treatment > > of HaCaT cells with 20 U/ml IFN-gamma for 24 h markedly induced > > adherence of Jurkat T cells to HaCaT cells. PYC pretreatment (50 > > microg/ml, 12 h) significantly inhibited IFN-gamma induced adherence of > > T cells to HaCaT cells (p < .01). ICAM-1 plays a major role in the > > IFN-gamma-induced adherence of T cells to keratinocytes. Thus, the > > effect of PYC on IFN-gamma-induced ICAM-1 expression was investigated as > > well. Pretreatment of HaCaT cells with PYC significantly inhibited > > IFN-gamma-induced expression of ICAM-1 expression in HaCaT cells. The > > downregulation of inducible ICAM-1 expression by PYC was both dose and > > time dependent. A 50 microg/ml dose of PYC and a 12 h pretreatment time > > (i.e., before activation with IFN-gamma) provided maximal (approximately > > 70%) inhibition of inducible ICAM-1 expression in HaCaT cells. > > Gamma-activated sequence present on the ICAM-1 gene confers IFN-gamma > > responsiveness in selected cells of epithelial origin (e.g., > > keratinocytes) that are known to express ICAM-1 on activation with > > IFN-gamma. Gel-shift assays revealed that PYC inhibits > > IFN-gamma-mediated activation of Stat1, thus suggesting a > > transcriptional regulation of inducible ICAM-1 expression by PYC. These > > results indicate the therapeutic potential of PYC in patients with > > inflammatory skin disorders.
– "Don’t wait for a light to appear at the end of the tunnel. Stride down there and light the bloody thing yourself!" Web sites at http://www.jaragun.com/ http://www.geocities.com/peripata/
Response:
What if you’re scared of needles? Carmel jack n dalton wrote: > I think this means that pycnogenol(Pine Bark extract) fights Interferon > Gamma induced inflammation . Which sound good to me. See you all in the > forest. > Jack > P.S. Hint — They are the trees with the needles…
– "Don’t wait for a light to appear at the end of the tunnel. Stride down there and light the bloody thing yourself!" Web sites at http://www.jaragun.com/ http://www.geocities.com/peripata/
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